![]() ![]() The imaging platform consists of a customized box in which ears are repeatedly imaged as they rotate via motorized rollers. ![]() EARBOX integrates open-source technologies for both software and hardware that facilitate its deployment and improvement for specific research questions. We developed EARBOX, a low-cost, open-source system for automated phenotyping of maize ears. The lengths of the FZ, BA, and AA zones were measured manually along the main axis of the ear. Grey zones: basal (BA) and apical (AA) abortion zones, where the surface occupied by grains visually represents less than 50% of the visible surface of the ear. White zone: fertile zone of the ear (FZ), where the surface occupied by grains visually represents more than 50% of the visible surface of the ear. ![]() ![]() The measurement was repeated 4 times around the ear. Green areas: example of grains used for manual phenotyping of the average number of cohorts per ear (CN). To standardize the measurement, the method consisted in counting the number of rows (= lines of grains along the ear perpendicular to the cohorts) with at least one grain per row for each third of the ear. Red areas: example of grains used for manual phenotyping of basal (BGC), median (MGC) and apical (AGC) number of grains per cohort, particularly difficult to characterize on "scattered" ears. (B) Sample ear from the environmental diversity panel grown under water deficit conditions showing partial pollination and/or ovary and grain abortion. (A) Sample ear from the biological diversity panel. Methodology for manual phenotyping of maize ears. The Creative Commons Public Domain Dedication waiver ( ) applies to the data made available in this article, unless otherwise stated in a credit line to the data.Īdditional file 3 Fig. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. ![]()
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